Fluorescent Sensors

Digital fluorescent pH sensors.

We designed polymeric sensors that created a digital-type fluorescence response to pH variation in an aqueous solution.

Fluorescent Sensors for Biological Applications

Fluorescence is one of the most important analytical methods used in biological studies. In the past decade or two, instrumentation in this field has greatly advanced, and now it is possible to detect single photons or fluorescent molecules [1,2], or break the Abbe diffraction limit to distinguish two points spaced less than 50 nm apart [3]. Concurrently, the development of improved fluorescent...

Thioamide-Based Fluorescent Protease Sensors

Thioamide quenchers can be paired with compact fluorophores to design "turn-on" fluorescent protease substrates. We have used this method to study a variety of serine-, cysteine-, carboxyl-, and metallo-proteases, including trypsin, chymotrypsin, pepsin, thermolysin, papain, and calpain. Since thioamides quench some fluorophores red-shifted from those naturally occurring in proteins, this techn...

Benzobisoxazole cruciforms as fluorescent sensors.

CONSPECTUS: Cross-conjugated molecular cruciforms are intriguing platforms for optoelectronic applications. Their two intersecting π-conjugated arms allow independent modulation of the molecules' HOMO and LUMO levels and guarantee a well-defined optical response to analyte binding. In addition, the rigid cross-conjugated geometries of these molecules allow their organization in two- and three-d...

Long-Wavelength, Reaction-Based, Fluorescent Sensors